Man pages for keshav-motwani/scanalysis
Multi-Sample Visualization and Immune Repertoire Analysis Utilities for Single-Cell Data

annotate_ambient_profileAdd average of ambient feature reads to rowData
annotate_cdr3Annotate CDR3 sequences stored in colData(sce)$vdj based on a...
annotate_chain_countAnnotate number of TRA, TRB, IGL, IGK, and IGH reads per...
annotate_clonotype_countAnnotate number of occurrences of a given clonotype in a...
annotate_n_cells_exprAdd number of cells with nonzero expression for each gene to...
annotate_n_genes_exprAdd number of genes expressed per barcode to...
annotate_pct_gene_setAdd percent of gene set defined by a regular expression...
annotate_pct_total_readsAdd percentage of total reads that each gene takes up across...
annotate_total_umi_countAdd number of total umis per barcode to...
assign_clonotypesFunction to use for assigning clonotypes based on custom...
cacheCache and retrieve intermediate steps if path exists
compute_evenness_profile_longCompute tidied data frame of evenness profiles per group...
compute_evenness_profile_matrixCompute matrix of evenness profiles per group based on...
convert_identity_frequency_matrix_to_longConvert an identity frequency matrix
dot-encode_identity_frequency_matrixHelper function to encode identity frequency matrix given...
dot-filter_ambient_barcode_cellrangerGet filter for cells based on inflection point
dot-filter_ambient_barcode_empty_dropsGet filter for cells based on emptyDrops algorithm
dot-filter_ambient_barcode_inflectionGet filter for cells based on inflection point
dot-filter_ambient_barcode_kneeGet filter for cells based on knee point
dot-get_cell_annotationsGet cell annotations for feature_heatmap
dot-get_cell_features_assayGet feature from assay (from alternate experiment)
dot-get_cell_features_assay_explicit_expGet feature from assay (from alternate experiment)
dot-get_cell_features_coldataGet feature from column metadata
dot-get_cell_features_reduced_dimensionsGet feature from reduced dimensional representation
dot-get_feature_annotationsGet feature annotations for feature_heatmap
dot-get_reduced_dimsGet reduced dimensions of object
dot-prepare_barcode_countsPrepare count annotations for number of cells passing each...
dot-prepare_barcode_cutoffsPrepare cutoff values for each method
dot-prepare_barcode_plot_dataPrepare data for plotting barcode QC plot
dot-prepare_gex_bivariate_countsPrepare counts of cells that meet filter criteria to annotate...
dot-prepare_gex_dataPrepare data frame to plot for gene expression related QC...
dot-prepare_gex_scale_limitsPrepare the limits of plot scale
dot-prepare_gex_univariate_countsPrepare counts of cells that meet filter criteria to annotate...
dot-prepare_volcano_annotationsPrepare annotations for volcano plot
dot-read_10x_gene_exprRead gene expression matrix from 10X data
dot-read_10x_vdjRead VDJ information from 10X data
dot-sce_to_assayConvert SingleCellExperiment object to Seurat assay
dot-seurat_assay_to_sceConvert Seurat assay to SingleCellExperiment
encode_cell_identity_frequency_longEncode frequencies of (combinations) of values in columns...
encode_cell_identity_frequency_matrixEncode frequencies of (combinations) of values in columns...
encode_vdj_identity_frequency_longEncode frequencies of (combinations) of values in columns...
encode_vdj_identity_frequency_matrixEncode frequencies of (combinations) of values in columns...
filter_ambient_barcodeGet filter to remove ambient RNA barcodes based on the...
filter_n_genes_exprGet filter for number of genes expressed per barcode based on...
filter_pct_mitoGet filter for percentage of mitochondrial reads expressed...
filter_total_umiGet filter for total umis per barcode based on log...
filter_vdj_chain_countGet filter for barcodes based on the number of chains for...
get_assay_dataGet assay data from either the main experiment or altExps
get_cell_featuresGet feature from assay data, colData, or reducedDims at once...
get_multi_sample_pbmc_10kGet modified version of PBMC 10k data from 10X
get_pbmc_5k_nextgemGet PBMC 5k data using v3 chemistry
get_pbmc_5k_v3Get PBMC 5k data using v3 chemistry
get_row_dataGet rowData from either the main experiment or altExps
pipePipe operator
plot_barcode_qcQC plot of UMI rank vs total number of UMIs per barcode
plot_feature_distributionsPlot feature distributions from SingleCellExperiment objects
plot_feature_heatmapPlot heatmap of features across cells with annotations
plot_gex_bivariate_qcScatterplot of two features of interest from colData with...
plot_gex_univariate_qcScatterplot of two features of interest from colData with...
plot_pairwise_featuresPlot pairwise scatterplot of cell-level data
plot_reduced_dimensionsPlot reduced dimensional plot with multiple features
plot_vdj_gex_univariate_qcScatterplot of two features of interest from colData with...
plot_volcanoPlot volcano plot with annotations
read_10xRead 10X gene expression and VDJ data into a...
sce_to_seuratConvert SingleCellExperiment object to Seurat and retain...
select_top_de_genesSelect the top DE genes, ranked on either fold change or...
seurat_to_sceConvert Seurat object to SingleCellExperiment and retain...
unnest_vdjGet VDJ data with cell-level metadata
keshav-motwani/scanalysis documentation built on Feb. 4, 2021, 2:21 a.m.