annotate.binding.events: Annotation of Primer Binding Events.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Annotation of Primer Binding Events.

Description

Annotates whether primer binding events are in the allowed binding region or not.

Usage

annotate.binding.events(
  fw.binding,
  allowed.range,
  nbr.primers,
  allowed.region.definition = c("within", "any")
)

Arguments

fw.binding	IRanges with coverage information.
allowed.range	IRanges of the allowed binding ranges in the templates.
nbr.primers	Number of primers to consider.
allowed.region.definition	Definition of the allowed binding region

Value

IRanges with annotations of (preliminary) specificity and allowed binding. The field all_binding contains all binding regions, on_target contains all events in the target region, and off_target contains all off-target binding events.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.