check_cvg_constraints: Evaluation of Coverage Constraints.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Evaluation of Coverage Constraints.

Description

Computes the biochemical properties specified in the settings object and determines whether the primers fulfill the required constraints.

Usage

check_cvg_constraints(
  primer.df,
  template.df,
  settings,
  active.constraints = names(cvg_constraints(settings)),
  to.compute.constraints = active.constraints,
  for.shiny = FALSE,
  updateProgress = NULL
)

Arguments

primer.df	A Primers object containing the primers to be checked.
template.df	A Templates object containing the template sequences corresponding to the primers.
settings	A DesignSettings object containing the coverage constraints to be checked and their settings.
active.constraints	Identifiers of constraints that are to be checked.
to.compute.constraints	Constraints that are to be computed.
for.shiny	Whether to format output for HTML.
updateProgress	Progress callback function for shiny.

Value

A Primers object with with columns for each constraint in active.constraints.

Note

Please note that some constraints can only be computed if additional software is installed, please see DesignSettings for an overview.

matdoering/openPrimeR documentation built on Feb. 11, 2024, 9:22 p.m.