assign_binding_regions.character: Character Assignment of Binding Regions

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Character Assignment of Binding Regions

Description

Generic method for assigning the binding region using individual binding regions.

Usage

## S3 method for class 'character'
assign_binding_regions(
  template.df,
  fw,
  rev,
  optimize.region = FALSE,
  primer.length = 20,
  gap.char = "-"
)

Arguments

template.df	Template data frame.
fw	FASTA file specifying the forward binding regions.
rev	FASTA file specifying the reverse binding regions.
optimize.region	Should the primer binding region be optimized using secondary structure prediction?
primer.length	Probe length for optimizing template secondary structure.
gap.char	The gap character for aligned sequences.

Value

Template data frame with assigned binding regions.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.