cascaded.filter | R Documentation |
Filter primers according to constraints and relax constraints if necessary.
cascaded.filter(
primer.df,
template.df,
settings,
mode.directionality = c("fw", "rev", "both"),
required.cvg = 1,
target.temps = NULL,
updateProgress = NULL,
results.loc = NULL
)
primer.df |
Primer data frame. |
template.df |
Template data frame. |
settings |
Settings object. |
mode.directionality |
Primer direction. |
required.cvg |
Required ratio of covered templates.
If |
target.temps |
Target melting temperature of the primers in Celsius. This argument is only required if we try to match the melting temperatures of another primer set, e.g. when first optimizing forward and then optimizing reverse primers. |
updateProgress |
Progress callback function for shiny. |
results.loc |
Directory where the filtering results should be stored. |
Constraints are relaxed if the required.cvg
could not be reached with the input constraints.
The filtered primer data frame with respect to required.cvg
.
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