call.melt.single: Thermodynamic melting temperature computations.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Thermodynamic melting temperature computations.

Description

Computes the melting temperature for the input primers.

Usage

call.melt.single(
  primers,
  complements,
  out.file,
  primer_conc,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  ID
)

Arguments

primers	List of primer strings.
complements	List with corresponding complements.
out.file	Path to the file where MELTING will write the results.
primer_conc	Primer concentration.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.
ID	identifiers of the input primers

Value

Melting temperature data frame.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.