call.melt: Thermodynamic melting temperature computations.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Thermodynamic melting temperature computations.

Description

Computes the melting temperature for the input primers.

Usage

call.melt(
  primers,
  complements,
  primer_conc,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc
)

Arguments

primers	Character vector of primer strings.
complements	Character vector with complement sequences corresponding to primers.
primer_conc	Primer concentration.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.

Value

Melting temperature data frame.

References

Le Novère N. (2001). MELTING, computing the melting temperature of nucleic acid duplex. Bioinformatics, 17: 1226-1227. Dumousseau M., Rodriguez N., Juty N., Le Novère N. (2012) MELTING, a flexible platform to predict the melting temperatures of nucleic acids. BMC Bioinformatics, 13: 101.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.