See convert_motifs() for acceptable motif formats.
use.type
character(1) One of c('PCM', 'PPM', 'PWM', 'ICM').
method
character(1) One of PCC, EUCL, SW, KL, ALLR, BHAT, HELL,
SEUCL, MAN, ALLR_LL, WEUCL, WPCC. See details.
tryRC
logical(1) Try the reverse complement of the motifs as well,
report the best score.
min.overlap
numeric(1) Minimum overlap required when aligning the
motifs. Setting this to a number higher then the width of the motifs
will not allow any overhangs. Can also be a number between 0 and 1,
representing the minimum fraction that the motifs must overlap.
min.mean.ic
numeric(1) Minimum mean information content between the
two motifs for an alignment to be scored. This helps prevent scoring
alignments between low information content regions of two motifs.
relative_entropy
logical(1) Change the ICM calculation affecting
min.position.ic and min.mean.ic. See convert_type().
normalise.scores
logical(1) Favour alignments which leave fewer
unaligned positions, as well as alignments between motifs of similar length.
Similarity scores are multiplied by the ratio of
aligned positions to the total number of positions in the larger motif,
and the inverse for distance scores.
min.position.ic
numeric(1) Minimum information content required between
individual alignment positions for it to be counted in the final alignment
score. It is recommended to use this together with normalise.scores = TRUE,
as this will help punish scores resulting from only a fraction of an
alignment.
score.strat
character(1) How to handle column scores calculated from
motif alignments. "sum": add up all scores. "a.mean": take the arithmetic
mean. "g.mean": take the geometric mean. "median": take the median.
"wa.mean", "wg.mean": weighted arithmetic/geometric mean. "fzt": Fisher
Z-transform. Weights are the
total information content shared between aligned columns.
return.raw
logical(1) Instead of returning a plot, return the
aligned named matrices used to generate the plot. This can be useful
if you wish to use view_motifs() alignment capabilities for custom
plotting uses. Alignment is performed by adding empty columns to the
left or right of motifs to generate matrices of equal length.
dedup.names
logical(1) Plotting motifs with duplicated names is
not allowed. Setting this to TRUE allows the names to be modified
for plotting.
show.positions
logical(1) Show x-axis position tick labels.
show.positions.once
logical(1) When plotting multiple motifs,
show x-axis position tick labels only once.
show.names
logical(1) Add motif names when plotting multiple
motifs.
...
Additional options for ggseqlogo::geom_logo().
Details
Since the ggseqlogo package can only plot individual
characters and not strings, plotting the multifreq slot is not
supported. See the examples section for plotting the multifreq
slot using the Logolas package.
See compare_motifs() for more info on comparison parameters.
Note: score.strat = "a.mean" is NOT recommended, as view_motifs() will
not discriminate between two alignments with equal mean scores, even if one
alignment is longer than the other.