normQpcrQuantile: Function for Quantile Normalization of qPCR Data.

Description Usage Arguments Value Author(s) See Also Examples

Description

Implements quantile normalization for a qpcrBatch object. We have adapted this algorithm from the function normalizeBetweenArrays from the limma package.

Data in a qpcrBatch object is normalized such that within an experiment, the expression distributions

across plates are more or less identical, and across experiments, the expression distributions

are also now more or less identical.

Usage

1

Arguments

qBatch

A link{qpcrBatch} object.

Value

A link{qpcrBatch} object, the normalized slot is now set at TRUE.

Author(s)

Jess Mar jess@jimmy.harvard.edu

See Also

normQpcrRankInvariant, normalizeBetweenArrays

Examples

1
2

Example output

Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: limma

Attaching package: 'limma'

The following object is masked from 'package:BiocGenerics':

    plotMA

Loading required package: affy
Warning message:
In read.dcf(con) :
  URL 'http://bioconductor.org/BiocInstaller.dcf': status was 'Couldn't resolve host name'

qpcrNorm documentation built on Nov. 8, 2020, 4:54 p.m.