qpcrBatch.object: qpcrBatch instance qpcrBatch.object

In qpcrNorm: Data-driven normalization strategies for high-throughput qPCR data.

Description

This is an artifically generated qPCR data set. The data set has been closely simulated from original data for 2396 genes on 13 time points. Each measurement within the one sample was repeated over three replicate wells, across multiple plates.

Usage

data(qpcrBatch.object)

Format

A data frame with 2396 observations on the following 41 variables.

Primers

Character vector of gene or primer pair names.

Plate_Index

Numeric vector denoting plate indices.

Time1_Rep1

Ct values for first time point, first replicate.

Time1_Rep2

Ct values for first time point, second replicate.

Time1_Rep3

Ct values for first time point, third replicate.

Examples

data(qpcrBatch.object)

Example output

Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, basename, cbind, colMeans, colSums, colnames,
    dirname, do.call, duplicated, eval, evalq, get, grep, grepl,
    intersect, is.unsorted, lapply, lengths, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind,
    rowMeans, rowSums, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unsplit, which, which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: limma

Attaching package: 'limma'

The following object is masked from 'package:BiocGenerics':

    plotMA

Loading required package: affy
Warning message:
In read.dcf(con) :
  URL 'http://bioconductor.org/BiocInstaller.dcf': status was 'Couldn't connect to server'

qpcrNorm documentation built on Nov. 8, 2020, 4:54 p.m.