tests/testthat/test_scan_sequences.R

In universalmotif: Import, Modify, and Export Motifs with R

context("scan_sequences()")

test_that("Results are accurate", {

  motif <- create_motif("AAAA", pseudocount = 1, nsites = 100)
  seq <- Biostrings::DNAStringSet("GGGAAAAGGGTTTTGGG")  # width 17
  res <- scan_sequences(motif, seq, RC = TRUE, verbose = 0)

  expect_equal(res$start[1], 4)
  expect_equal(res$motif[1], "motif")
  expect_equal(res$sequence[1], "1")
  expect_equal(res$stop[1], 7)
  expect_equal(res$match[1], "AAAA")
  expect_equal(res$strand[1], "+")
  expect_equal(res$score[1], 7.956, tolerance = 0.001)
  expect_equal(res$`max.score`[1], 7.957, tolerance = 0.001)
  expect_equal(res$`score.pct`[1], 100, tolerance = 0.1)

  expect_equal(res$score[1], res$score[2])
  expect_equal(res$`max.score`[1], res$`max.score`[2])
  expect_equal(res$strand[2], "-")
  expect_equal(res$start[2], 14)
  expect_equal(res$stop[2], 11)

  m <- create_motif(create_sequences(seqlen = 10), add.multifreq = 2, pseudocount = 1)
  s <- create_sequences()
  r <- scan_sequences(m, s, RC = TRUE, use.freq = 2, threshold = 0.8,
                      threshold.type = "logodds", verbose = 0)

  expect_true(is(r, "DataFrame"))

})