ReadVCFs: Read VCF files

In ICAMS: In-Depth Characterization and Analysis of Mutational Signatures ('ICAMS')

Description

Read VCF files

Usage

ReadVCFs(
  files,
  variant.caller = "unknown",
  num.of.cores = 1,
  names.of.VCFs = NULL,
  tumor.col.names = NA,
  filter.status = NULL,
  get.vaf.function = NULL,
  ...
)

Arguments

files	Character vector of file paths to the VCF files.
variant.caller	Name of the variant caller that produces the VCF, can be either "strelka", "mutect", "freebayes" or "unknown". This information is needed to calculate the VAFs (variant allele frequencies). If variant caller is "unknown"(default) and get.vaf.function is NULL, then VAF and read depth will be NAs. If variant caller is "mutect", do not merge SBSs into DBS.
num.of.cores	The number of cores to use. Not available on Windows unless num.of.cores = 1.
names.of.VCFs	Character vector of names of the VCF files. The order of names in names.of.VCFs should match the order of VCF file paths in files. If NULL(default), this function will remove all of the path up to and including the last path separator (if any) and file paths without extensions (and the leading dot) will be used as the names of the VCF files.
tumor.col.names	Optional. Only applicable to Mutect VCFs. Character vector of column names in Mutect VCFs which contain the tumor sample information. The order of names in tumor.col.names should match the order of Mutect VCFs specified in files. If tumor.col.names is equal to NA(default), this function will use the 10th column in all the Mutect VCFs to calculate VAFs. See GetMutectVAF for more details.
filter.status	The status indicating a variant has passed all filters. An example would be "PASS". Variants which don't have the specified filter.status in the FILTER column in VCF will be removed. If NULL(default), no variants will be removed from the original VCF.
get.vaf.function	Optional. Only applicable when variant.caller is "unknown". Function to calculate VAF(variant allele frequency) and read depth information from original VCF. See GetMutectVAF as an example. If NULL(default) and variant.caller is "unknown", then VAF and read depth will be NAs.
...	Optional arguments to get.vaf.function.

Value

A list of data frames storing data lines of the VCF files with two additional columns added which contain the VAF(variant allele frequency) and read depth information.

ICAMS documentation built on April 3, 2021, 5:07 p.m.