supermat: Build PHYLIP supermatrix and RAxML partition file using...

In phylotools: Phylogenetic Tools for Eco-Phylogenetics

Description

Build PHYLIP supermatrix and create RAxML partition file using aligned fasta or phylip files.

Usage

supermat(infiles, outfile = "supermat.out.phy",
         partition.file = "gene_partition.txt")

Arguments

infiles	a character string vector for phylip or aligned fasta file.
outfile	the name of the PHYLIP supermatrix
partition.file	partition data summary describing the genes.

Details

Supermatrix here means a phylip file with combined aligned sequences. The missing sequences should be replaced with either "?" or "-".

Value

A list containing: (1)supermat.dat:a list containing all the data frames read by read.phylip or read.fasta (2)res.super.dat: a data frame containing the sequences and the names (3)partition.dat: summary for all the fasta or phylip files (4)partition.dat.vector: character string vector for the partition file for RAxML

Note

Punctuation characters and white space in the names of the sequences will be replaced by "_". More information can be found at regex. Type of the sequence in the RAxML partition file should be changed manually according to the manual of RAxML.

Author(s)

Jinlong Zhang <[e-mail]>

References

Kress, W. J., Erickson, D. L., Jones, F. A., Swenson, N. G., Perez, R., Sanjur, O., & Bermingham, E. (2009). Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proceedings of the National Academy of Sciences, 106(44), 18621-18626.

de Queiroz, A.and Gatesy, J. (2007). The supermatrix approach to systematics. Trends in Ecology & Evolution, 22(1), 34-41.

https://github.com/stamatak/standard-RAxML

See Also

read.fasta,read.phylip,dat2phylip,

Examples

  cat("6 22",
  "seq_1    --TTACAAATTGACTTATTATA",
  "seq_2    GATTACAAATTGACTTATTATA",
  "seq_3    GATTACAAATTGACTTATTATA",
  "seq_5    GATTACAAATTGACTTATTATA",
  "seq_8    GATTACAAATTGACTTATTATA",
  "seq_10   ---TACAAATTGAATTATTATA",
  file = "matk.phy", sep = "\n")

  cat("5 15",
  "seq_1     GATTACAAATTGACT",
  "seq_3     GATTACAAATTGACT",
  "seq_4     GATTACAAATTGACT",
  "seq_5     GATTACAAATTGACT",
  "seq_8     GATTACAAATTGACT",
  file = "rbcla.phy", sep = "\n")

  cat("5 50",
  "seq_2          GTCTTATAAGAAAGAATAAGAAAG--AAATACAAA-------AAAAAAGA",
  "seq_3          GTCTTATAAGAAAGAAATAGAAAAGTAAAAAAAAA-------AAAAAAAG",
  "seq_5          GACATAAGACATAAAATAGAATACTCAATCAGAAACCAACCCATAAAAAC",
  "seq_8          ATTCCAAAATAAAATACAAAAAGAAAAAACTAGAAAGTTTTTTTTCTTTG",
  "seq_9          ATTCTTTGTTCTTTTTTTTCTTTAATCTTTAAATAAACCTTTTTTTTTTA",
  file = "trn1.phy", sep = "\n")

supermat(infiles = c("matk.phy", "rbcla.phy", "trn1.phy"))
unlink(c("matk.phy", "rbcla.phy", "trn1.phy"))
unlink(c("supermat.out.phy","gene_partition.txt"))

cat(
  ">seq_1",  "--TTACAAATTGACTTATTATA",
  ">seq_2",  "GATTACAAATTGACTTATTATA",
  ">seq_3",  "GATTACAAATTGACTTATTATA",
  ">seq_5",  "GATTACAAATTGACTTATTATA",
  ">seq_8",  "GATTACAAATTGACTTATTATA",
  ">seq_10", "---TACAAATTGAATTATTATA",
  file = "matk.fasta", sep = "\n")

cat(
  ">seq_1", "GATTACAAATTGACT",
  ">seq_3", "GATTACAAATTGACT",
  ">seq_4", "GATTACAAATTGACT",
  ">seq_5", "GATTACAAATTGACT",
  ">seq_8", "GATTACAAATTGACT",
  file = "rbcla.fasta", sep = "\n")

cat(
  ">seq_2", "GTCTTATAAGAAAGAATAAGAAAG--AAATACAAA-------AAAAAAGA",
  ">seq_3", "GTCTTATAAGAAAGAAATAGAAAAGTAAAAAAAAA-------AAAAAAAG",
  ">seq_5", "GACATAAGACATAAAATAGAATACTCAATCAGAAACCAACCCATAAAAAC",
  ">seq_8", "ATTCCAAAATAAAATACAAAAAGAAAAAACTAGAAAGTTTTTTTTCTTTG",
  ">seq_9", "ATTCTTTGTTCTTTTTTTTCTTTAATCTTTAAATAAACCTTTTTTTTTTA",
  file = "trn1.fasta", sep = "\n")

supermat(infiles = c("matk.fasta", "rbcla.fasta", "trn1.fasta"))
unlink(c("matk.fasta", "rbcla.fasta", "trn1.fasta"))

unlink(c("supermat.out.phy","gene_partition.txt"))

phylotools documentation built on May 2, 2019, 3:25 a.m.