bt.coverage: Returns the depth and breadth of coverage of features from B...

View source: R/bt.coverage.R

bt.coverageR Documentation

Returns the depth and breadth of coverage of features from B on the intervals in A.

Description

Returns the depth and breadth of coverage of features from B on the intervals in A.

Usage

bt.coverage(
  a,
  b,
  hist = NULL,
  d = NULL,
  counts = NULL,
  mean = NULL,
  s = NULL,
  S = NULL,
  f = NULL,
  F = NULL,
  r = NULL,
  e = NULL,
  split = NULL,
  g = NULL,
  nonamecheck = NULL,
  sorted = NULL,
  bed = NULL,
  header = NULL,
  nobuf = NULL,
  iobuf = NULL,
  output = NULL
)

Arguments

a

<bed/gff/vcf>

b

<bed/gff/vcf>

hist

Report a histogram of coverage for each feature in A as well as a summary histogram for _all_ features in A. Output (tab delimited) after each feature in A: 1) depth 2) # bases at depth 3) size of A 4) percent of A at depth

d

Report the depth at each position in each A feature. Positions reported are one based. Each position and depth follow the complete A feature.

counts

Only report the count of overlaps, don't compute fraction, etc.

mean

Report the mean depth of all positions in each A feature.

s

Require same strandedness. That is, only report hits in B that overlap A on the _same_ strand. - By default, overlaps are reported without respect to strand.

S

Require different strandedness. That is, only report hits in B that overlap A on the _opposite_ strand. - By default, overlaps are reported without respect to strand.

f

Minimum overlap required as a fraction of A. - Default is 1E-9 (i.e., 1bp). - FLOAT (e.g. 0.50)

F

Minimum overlap required as a fraction of B. - Default is 1E-9 (i.e., 1bp). - FLOAT (e.g. 0.50)

r

Require that the fraction overlap be reciprocal for A AND B. - In other words, if -f is 0.90 and -r is used, this requires that B overlap 90 percent of A and A _also_ overlaps 90 percent of B.

e

Require that the minimum fraction be satisfied for A OR B. - In other words, if -e is used with -f 0.90 and -F 0.10 this requires that either 90 percent of A is covered OR 10 percent of B is covered. Without -e, both fractions would have to be satisfied.

split

Treat "split" BAM or BED12 entries as distinct BED intervals.

g

Provide a genome file to enforce consistent chromosome sort order across input files. Only applies when used with -sorted option.

nonamecheck

For sorted data, don't throw an error if the file has different naming conventions for the same chromosome. ex. "chr1" vs "chr01".

sorted

Use the "chromsweep" algorithm for sorted (-k1,1 -k2,2n) input.

bed

If using BAM input, write output as BED.

header

Print the header from the A file prior to results.

nobuf

Disable buffered output. Using this option will cause each line of output to be printed as it is generated, rather than saved in a buffer. This will make printing large output files noticeably slower, but can be useful in conjunction with other software tools and scripts that need to process one line of bedtools output at a time.

iobuf

Specify amount of memory to use for input buffer. Takes an integer argument. Optional suffixes K/M/G supported. Note: currently has no effect with compressed files.

output

Output filepath instead of returning output in R.


PhanstielLab/bedtoolsr documentation built on Aug. 29, 2024, 5:37 p.m.