bt.multicov | R Documentation |
Counts sequence coverage for multiple bams at specific loci.
bt.multicov(
bams,
bed,
split = NULL,
s = NULL,
S = NULL,
f = NULL,
r = NULL,
q = NULL,
D = NULL,
F = NULL,
p = NULL,
output = NULL
)
bams |
aln.1.bam aln.2.bam ... aln.n.bam |
bed |
<bed/gff/vcf> |
split |
Treat "split" BAM or BED12 entries as distinct BED intervals. |
s |
Require same strandedness. That is, only report hits in B that overlap A on the _same_ strand. - By default, overlaps are reported without respect to strand. |
S |
Require different strandedness. That is, only report hits in B that overlap A on the _opposite_ strand. - By default, overlaps are reported without respect to strand. |
f |
Minimum overlap required as a fraction of each -bed record. - Default is 1E-9 (i.e., 1bp). - FLOAT (e.g. 0.50) |
r |
Require that the fraction overlap be reciprocal for each -bed and B. - In other words, if -f is 0.90 and -r is used, this requires that B overlap 90 percent of each -bed and the -bed record _also_ overlaps 90 percent of B. |
q |
Minimum mapping quality allowed. Default is 0. |
D |
Include duplicate reads. Default counts non-duplicates only |
F |
Include failed-QC reads. Default counts pass-QC reads only |
p |
Only count proper pairs. Default counts all alignments with MAPQ > -q argument, regardless of the BAM FLAG field. |
output |
Output filepath instead of returning output in R. |
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