This is a simpler subread aligner that runs of single fastq files rather than individual lane fastqs from the HiSeq. There maybe QC advantages of aligning the individual fastqc but sometimes it is just simpler to merge before the event.
1 2 3 4 5 6 | alignBWA(fileName, projName, runId, sampleBC = NULL,
outRoot = file.path("/scratch/pschofield/Projects", projName),
outName = "Data/alignments/bwa", noSub = F, scpIt = T,
bwaMod = "apps/bwa/0.7.13/gcc-4.4.7", debugRG = F,
samtoolsMod = "apps/samtools/0.1.19/gcc/4.4.7", mem = "32Gb",
refGenome = NULL, ncores = 8, fqExt = ".fastq", pe = T)
|
projName |
project name |
outRoot |
output root directory |
fileList |
list of fastq file |
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