call_variants_strelka: Call Somatic/Germline single nucleotide variants using...

Description Usage Arguments

View source: R/analysis.R

Description

Call Somatic/Germline single nucleotide variants using Strelka This function takes a pair of matched samples or single | multiple germline variants and calls variants on them. To specify the variant calling mode supply the right strelka binary. It is recommended to supply a vcf with indel candidates. This can be generated using MANTA workflow

Usage

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call_variants_strelka(
  bin_path = "tools/strelka-2.9.10/build/bin/configureStrelkaSomaticWorkflow.py",
  bin_path2 = "tools/manta-1.6.0/build/bin/configManta.py",
  bin_path3 = "tools/bcftools/bcftools",
  bin_path4 = "tools/htslib/bgzip",
  bin_path5 = "tools/htslib/tabix",
  tumor_bam = "",
  normal_bam = "",
  ref_genome = "",
  output_dir = "",
  patient_id = "",
  verbose = FALSE,
  targeted = FALSE,
  threads = 3,
  exec_options = "local"
)

Arguments

bin_path

REQUIRED Path to strelka binary. Somatic or Germline.

bin_path2

REQUIRED Path to manta binary. Somatic or Germline.

bin_path3

REQUIRED Path to bcftools binary.

bin_path4

REQUIRED Path to bgzip binary.

bin_path5

REQUIRED Path to tabix binary.

tumor_bam

REQUIRED Path to tumor bam file.

normal_bam

OPTIONAL Path to normal samples bam files.

ref_genome

REQUIRED Path to reference genome.

output_dir

OPTIONAL Path to the output directory.

patient_id

OPTIONAL Patient ID to name files after. If not given tumor_bam file name will be used, and if this is not given then normal_bam file will be used.

verbose

DEFAULT==FALSE Enables progress messages.

targeted

OPTIONAL If exome/capture method. Default FALSE

threads

OPTIONAL Number of threads per job. Default 3

exec_options

OPTIONAL Type of execution. local (Single node) / sge (multiple nodes). Default local.


TearsWillFall/variantCall documentation built on Oct. 15, 2021, 8:51 p.m.