Call Somatic/Germline single nucleotide variants using Strelka This function takes a pair of matched samples or single | multiple germline variants and calls variants on them. To specify the variant calling mode supply the right strelka binary. It is recommended to supply a vcf with indel candidates. This can be generated using MANTA workflow
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 | call_variants_strelka(
bin_path = "tools/strelka-2.9.10/build/bin/configureStrelkaSomaticWorkflow.py",
bin_path2 = "tools/manta-1.6.0/build/bin/configManta.py",
bin_path3 = "tools/bcftools/bcftools",
bin_path4 = "tools/htslib/bgzip",
bin_path5 = "tools/htslib/tabix",
tumor_bam = "",
normal_bam = "",
ref_genome = "",
output_dir = "",
patient_id = "",
verbose = FALSE,
targeted = FALSE,
threads = 3,
exec_options = "local"
)
|
bin_path |
REQUIRED Path to strelka binary. Somatic or Germline. |
bin_path2 |
REQUIRED Path to manta binary. Somatic or Germline. |
bin_path3 |
REQUIRED Path to bcftools binary. |
bin_path4 |
REQUIRED Path to bgzip binary. |
bin_path5 |
REQUIRED Path to tabix binary. |
tumor_bam |
REQUIRED Path to tumor bam file. |
normal_bam |
OPTIONAL Path to normal samples bam files. |
ref_genome |
REQUIRED Path to reference genome. |
output_dir |
OPTIONAL Path to the output directory. |
patient_id |
OPTIONAL Patient ID to name files after. If not given tumor_bam file name will be used, and if this is not given then normal_bam file will be used. |
verbose |
DEFAULT==FALSE Enables progress messages. |
targeted |
OPTIONAL If exome/capture method. Default FALSE |
threads |
OPTIONAL Number of threads per job. Default 3 |
exec_options |
OPTIONAL Type of execution. local (Single node) / sge (multiple nodes). Default local. |
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