makeIncucyteDRCSet: makeIncucyteDRCSet

In chapmandu2/IncucyteDRC: Dose Response Curves from Incucyte Proliferation Assays

Description

Function to construct an IncucyteDRCSet object. Contains data for just those wells that are from the same cell line background - ie cell line, passage number, cell number etc are all the same. Give that the same cell line background is used, the control wells are common to all wells regardless of different compounds or concentrations of compound.

Usage

makeIncucyteDRCSet(platemap, platedata, cut_time = NULL, metadata = NULL,
  pm_warn = TRUE)

Arguments

platemap	Platemap dataframe containing information for just those wells required
platedata	IncucyteDRCPlateData object that will be filtered according to the wells present in the platemap
cut_time	The time at which to extract the data for the dose response curve. Default is NULL.
metadata	A single row data frame containing any information specific to this IncucyteDRCSet object.
pm_warn	Boolean to control platemap check warnings. Default is TRUE.

Value

IncucyteDRCSet object

Examples

pm_file <- system.file(file='extdata/example.PlateMap', package='IncucyteDRC')
test_pm <- importPlatemapXML(pm_file)
data_file <- system.file(file='extdata/example_data.txt', package='IncucyteDRC')
test_data <- importIncucyteData(data_file, metric='pc')

test_pm_filtered <-  dplyr::filter(test_pm,
                                   samptype %in% c('C', 'S') & growthcondition == '8 x 10e4/mL')
test_set <- makeIncucyteDRCSet(test_pm_filtered, test_data)

print(test_set)

chapmandu2/IncucyteDRC documentation built on May 13, 2019, 3:28 p.m.