makeIncucyteDRCSet: makeIncucyteDRCSet

Description Usage Arguments Value Examples

Description

Function to construct an IncucyteDRCSet object. Contains data for just those wells that are from the same cell line background - ie cell line, passage number, cell number etc are all the same. Give that the same cell line background is used, the control wells are common to all wells regardless of different compounds or concentrations of compound.

Usage

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makeIncucyteDRCSet(platemap, platedata, cut_time = NULL, metadata = NULL,
  pm_warn = TRUE)

Arguments

platemap

Platemap dataframe containing information for just those wells required

platedata

IncucyteDRCPlateData object that will be filtered according to the wells present in the platemap

cut_time

The time at which to extract the data for the dose response curve. Default is NULL.

metadata

A single row data frame containing any information specific to this IncucyteDRCSet object.

pm_warn

Boolean to control platemap check warnings. Default is TRUE.

Value

IncucyteDRCSet object

Examples

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pm_file <- system.file(file='extdata/example.PlateMap', package='IncucyteDRC')
test_pm <- importPlatemapXML(pm_file)
data_file <- system.file(file='extdata/example_data.txt', package='IncucyteDRC')
test_data <- importIncucyteData(data_file, metric='pc')

test_pm_filtered <-  dplyr::filter(test_pm,
                                   samptype %in% c('C', 'S') & growthcondition == '8 x 10e4/mL')
test_set <- makeIncucyteDRCSet(test_pm_filtered, test_data)

print(test_set)

chapmandu2/IncucyteDRC documentation built on May 13, 2019, 3:28 p.m.