find_subseq_target_temp: Find a subsequence with a target PCR temperature.
In elsayed-lab/hpgltools: A pile of (hopefully) useful R functions
find_subseq_target_temp R Documentation
Find a subsequence with a target PCR temperature.
Description
Given a relatively large sequence, this function will iteratively
remove a single nucleotide and recalulate the TM until the TM falls
to the target temperature.
Usage
find_subseq_target_temp(
sequence,
target = 53,
direction = "forward",
verbose = FALSE
)
Arguments
sequence
Starting sequence.
target
Desire TM of the final sequence.
direction
What strand is expected for annealing this primer?
verbose
Be chatty?
elsayed-lab/hpgltools documentation built on May 9, 2024, 5:02 a.m.
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| find_subseq_target_temp | R Documentation |
Find a subsequence with a target PCR temperature.
Description
Given a relatively large sequence, this function will iteratively remove a single nucleotide and recalulate the TM until the TM falls to the target temperature.
Usage
find_subseq_target_temp(
sequence,
target = 53,
direction = "forward",
verbose = FALSE
)
Arguments
sequence |
Starting sequence. |
target |
Desire TM of the final sequence. |
direction |
What strand is expected for annealing this primer? |
verbose |
Be chatty? |
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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