compute.Ta: Annealing temperature

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Annealing temperature

Description

Computes the annealing temperature using all binding events.

Usage

compute.Ta(
  primer.df,
  template.df,
  mode.directionality = c("fw", "rev", "both"),
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  primer_conc
)

Arguments

primer.df	Primer data frame.
template.df	Template data frame
mode.directionality	Primer directionality.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.
primer_conc	Primer concentration.

Value

All annealing temperatures for given binding events.

References

Rychlik, W. J. S. W., W. J. Spencer, and R. E. Rhoads. "Optimization of the annealing temperature for DNA amplification in vitro." Nucleic acids research 18.21 (1990): 6409-6412.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.