compute.Tm.baldino: Baldino Formula
In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

View source: R/con_melting_temperature.R

compute.Tm.baldino

R Documentation

Baldino Formula

Description

Computes the melting temperature using the formulation by Baldino.

Usage

compute.Tm.baldino(
  sequences,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  primer_conc
)

Arguments

`sequences`	Input sequence strings.
`na_salt_conc`	Sodium ion concentration.
`mg_salt_conc`	Magensium ion concentration.
`k_salt_conc`	Potassium ion concentration.
`tris_salt_conc`	Tris buffer concentration.
`primer_conc`	Primer concentration.

Value

The melting temperature for the input sequences.

References

Rychlik, W. J. S. W., W. J. Spencer, and R. E. Rhoads. "Optimization of the annealing temperature for DNA amplification in vitro." Nucleic acids research 18.21 (1990): 6409-6412.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.