compute.all.cross.dimers: Cross dimerization

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Cross dimerization

Description

Compute worst-case DeltaG data frame with all possible primer cross-dimers.

Usage

compute.all.cross.dimers(
  primer.df,
  primer_conc,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  annealing.temp,
  results = NULL,
  check.idx = NULL,
  for.shiny = FALSE,
  no.structures = FALSE
)

Arguments

primer.df	Input primers data frame.
primer_conc	Primer concentration.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.
annealing.temp	The PCR annealing temperature.
results	(optional) Cross dimer data frame (unfiltered)
check.idx	Indices of primers for checking cross-dimerization.
for.shiny	Whether the table is inteded for HTML display.
no.structures	Whether dimerization structures shall not be outputted.

Value

Worst-case cross dimers.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.