compute.covered.Ta: Annealing temperature
In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

View source: R/con_annealing_temperature.R

compute.covered.Ta

R Documentation

Annealing temperature

Description

Computes the annealing temperature using all binding events.

Usage

compute.covered.Ta(
  primer.df,
  mode.directionality = c("fw", "rev", "both"),
  template.df,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  primer_conc
)

Arguments

`primer.df`	Primer data frame.
`mode.directionality`	Primer directionality.
`template.df`	Template data frame
`na_salt_conc`	Sodium ion concentration.
`mg_salt_conc`	Magensium ion concentration.
`k_salt_conc`	Potassium ion concentration.
`tris_salt_conc`	Tris buffer concentration.
`primer_conc`	Primer concentration.