compute.melting.temps: Computation of Melting Temperatures.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Computation of Melting Temperatures.

Description

Use nearest-neighbor thermodynamic computations to find melting temperatures.

Usage

compute.melting.temps(
  primer.df,
  primer_conc,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  mode.directionality = c("fw", "rev", "both")
)

Arguments

primer.df	Primer data frame.
primer_conc	Primer concentration.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.
mode.directionality	Direction of primers

Value

Data frame with melting temperature info for the input primers.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.