compute.sodium.equivalent.conc: Sodium-equivalent Concentration
In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

View source: R/con_melting_temperature.R

compute.sodium.equivalent.conc

R Documentation

Sodium-equivalent Concentration

Description

Computes the sodium-equivalent concentration for the input ion concentrations.

Usage

compute.sodium.equivalent.conc(
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc
)

Arguments

`na_salt_conc`	Sodium ion concentration.
`mg_salt_conc`	Magensium ion concentration.
`k_salt_conc`	Potassium ion concentration.
`tris_salt_conc`	Tris buffer concentration.

Value

The sodium-equivalent concentration of the input ion concentrations.

References

Record, M. Thomas. "Effects of Na+ and Mg++ ions on the helix–coil transition of DNA." Biopolymers 14.10 (1975): 2137-2158.

Owczarzy, Richard, et al. "Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations." Biochemistry 47.19 (2008): 5336-5353.

Peyret, Nicolas. Prediction of nucleic acid hybridization: parameters and algorithms. Detroit: Wayne State University, 2000.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.