compute_annealing_temp: Annealing temperature.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Annealing temperature.

Description

Identifies the optimal annealing temperature of a set of primers. If primers cover template sequences, the annealing temperature is computed using Rychlik's formula. Otherwise, the annealing temperature is determined using the rule of thumb based on the melting temperatures of the primers.

Usage

compute_annealing_temp(
  primer.df,
  mode.directionality,
  template.df,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc,
  primer_conc
)

Arguments

primer.df	Primer data frame.
mode.directionality	Primer directionality.
template.df	Template data frame
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris buffer concentration.
primer_conc	Primer concentration.

Value

The optimal annealing temperature.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.