create.initial.primer.set: Creation of an Initial Primer Set.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Creation of an Initial Primer Set.

Description

Creates an initial set of candidate primers for primer design.

Usage

create.initial.primer.set(
  template.df,
  primer.lengths,
  mode.directionality = c("fw", "rev"),
  sample,
  allowed.region.definition = c("within", "any"),
  init.algo = c("naive", "tree"),
  max.degen,
  conservation,
  updateProgress = NULL
)

Arguments

template.df	Template data frame.
primer.lengths	Vector containing the permissible primer lengths.
mode.directionality	Direction of primers to be created.
sample	Name of the template sample.
allowed.region.definition	Definition of the allowed binding region.
init.algo	Algorithm for initializing primers.
max.degen	Maximal allowed degeneration of created primers.
conservation	Required conservation of primers. The value of conservation should be in the range[0,1].
updateProgress	Shiny progress object.

Value

An initialized data frame of candidate primers.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.