create.primers.naive: Naive Initialization of Primers.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Naive Initialization of Primers.

Description

Initialize primers by extracting substrings from all templates.

Usage

create.primers.naive(
  seqs,
  seq.IDs,
  seq.groups,
  l.s,
  e.s,
  primer.lengths,
  allowed.region.definition,
  max.degen,
  sample = "",
  identifier = "",
  updateProgress = NULL
)

Arguments

seqs	The template sequence strings.
seq.IDs	The identifiers of the templates.
seq.groups	The group identifiers of the templates.
l.s	The positions where the allowed region starts for each template.
e.s	The positions where the allowed reigon ends for each template.
primer.lengths	Vector of desired primer lengths.
allowed.region.definition	Definition of the allowed region.
max.degen	Maximum allowed degeneracy of primers.
sample	Template sample identifier.
updatProgress	Shiny progress object.

Value

Data frame with initialized primer candidates.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.