filter.primer.set.opti: Filtering of Primers

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Filtering of Primers

Description

Filters a primer set during the optimization procedure.

Usage

filter.primer.set.opti(
  primer.df,
  sample,
  template.df,
  settings,
  mode.directionality,
  required.cvg,
  results.loc,
  target.temps
)

Arguments

primer.df	Primer data frame.
sample	Name of the current template sample.
template.df	Template data frame.
settings	List with settings for the constraints to be used for filtering.
mode.directionality	Primer direction.
required.cvg	Required ratio of covered templates. If required.cvg is set to 0, the constraints are not relaxed.
results.loc	Path to a directory where the results should be written.
target.temps	Target melting temperature of the primers in Celsius. This argument is only required if we try to match the melting temperatures of another primer set, e.g. when first optimizing forward and then optimizing reverse primers.

Value

The filtered primer data frame with respect to required.cvg.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.