get.cross.dimers: Cross dimers

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Cross dimers

Description

Computes all possible primer cross-dimers.

Usage

get.cross.dimers(
  primers.1,
  primers.2,
  ions,
  annealing.temp,
  check.idx = NULL,
  no.structures = FALSE,
  mode = c("symmetric", "asymmetric")
)

Arguments

primers.1	Input primers.
primers.2	Input primers.
ions	Sodium-equivalent ionic concentration.
annealing.temp	The PCR annealing temperature.
check.idx	indices of primers for checking cross-dimerization
no.structures	Whether to compute structures of dimers.
mode	'symmetric', if primers.1 and primers.2 carry the same information (i.e. fw-fw, rev-rev, fw-rev), 'asymetric' else.

Value

Data frame with potential cross dimers.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.