get.cvg.constraint.settings: Gather all Coverage Constraints.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Gather all Coverage Constraints.

Description

Constructor for coverage constraint settings.

Usage

get.cvg.constraint.settings(
  allowed.stop.codons,
  allowed.efficiency,
  disallowed.mismatch.pos,
  allowed.anneal.deltaG,
  allowed.substitutions,
  allowed.coverage.model
)

Arguments

allowed.stop.codons	Whether mismatch binding events inducing stop codons in the amino acid sequence are allowed.
allowed.efficiency	Min/max for primer efficiency.
disallowed.mismatch.pos	The positions from the 3' terminal end of primers where mismatches shall be prevented.
allowed.anneal.deltaG	Maximal allowed free energy of template-primer annealing.
allowed.substitutions	Whether mismatch binding events inducing substitutions in the amino acid sequence are allowed.

Value

List with all coverage constraint settings.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.