get.duplex.energies: Determination of the Free Binding Energy.

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Determination of the Free Binding Energy.

Description

Computest the free energy of annealing between primers and templates. If the mode is set to "on_target", the free energies of binding events in the allowed region are computed, while if the mode is set to "off_target", the free energies of off-target events are computed.

Usage

get.duplex.energies(
  primer.df,
  template.df,
  annealing.temp,
  settings,
  mode = c("on_target", "off_target")
)

Arguments

primer.df	A Primers object.
template.df	A Templates object.
annealing.temp	The vector of optimal annealing temperatures of the primers.
settings	A DesignSettings object.
mode	If the mode is set to "on_target", the free energies of binding events in the allowed region are computed, while if the mode is set to "off_target", the free energies of off-target events are computed.

Value

A list of lists containing the numeric free energies of the annealing events for every primer.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.