initialize.primer.set: Creation of Initial Primers

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Creation of Initial Primers

Description

Creates a set of candidate primers.

Usage

initialize.primer.set(
  template.df,
  sample.name,
  primer.lengths,
  allowed.region.definition,
  mode.directionality,
  init.algo,
  max.degen,
  conservation,
  cur.results.loc
)

Arguments

template.df	Template data frame.
sample.name	Name of the template sample.
primer.lengths	Interval of minimal and maximal desired primer length.
allowed.region.definition	Definition of the allowed binding region.
mode.directionality	Direction of primers to be created.
init.algo	Algorithm for initializing primers.
max.degen	Maximal allowed degeneration of created primers.
conservation	Required conservation of primers. The value of conservation should be in the range[0,1].
cur.results.loc	Location for writing the primers as csv.

Value

An initial primer data frame.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.