relax.constraints: Relaxation of Constraints

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Relaxation of Constraints

Description

Relax constraints trying to reach the target coverage ratio.

Usage

relax.constraints(
  settings,
  filtered.df,
  excluded.df,
  stat.df,
  template.df,
  mode.directionality = c("fw", "rev"),
  required.cvg,
  target.temps = NULL,
  results.loc = NULL
)

Arguments

settings	A DesignSettings object.
filtered.df	Data set of primers that fulfilled all constraints of the filtering procedure.
excluded.df	Data frame with excluded primers from the first filtering round.
stat.df	Data frame with statistics from filtering.
template.df	Template data frame.
mode.directionality	Primer direction.
required.cvg	Required ratio of covered templates.
target.temps	Target melting temperature values.
results.loc	The location where intermediary results should be stored.

Value

A list containing information about the relaxation as well as the filtered primers.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.