select.best.primer.idx: Greedy Choice

In matdoering/openPrimeR: Multiplex PCR Primer Design and Analysis

Greedy Choice

Description

Selects the currently best primer for Greedy primer selection.

Usage

select.best.primer.idx(
  result,
  primers,
  deltaG.cutoff,
  target.temp,
  primer_conc,
  na_salt_conc,
  mg_salt_conc,
  k_salt_conc,
  tris_salt_conc
)

Arguments

result	Data frame of current optimized primer data set that is to be augmented.
primers	Data frame of candidate primers for addition to result.
deltaG.cutoff	Free energy cutoff for cross-dimerization.
target.temp	Target annealing temperature in Celsius.
primer_conc	Primer concentration.
na_salt_conc	Sodium ion concentration.
mg_salt_conc	Magensium ion concentration.
k_salt_conc	Potassium ion concentration.
tris_salt_conc	Tris ion concentration.

Value

The index of a suitable primer according to Greedy selection.

matdoering/openPrimeR documentation built on July 4, 2025, 3:59 a.m.