compareSimulated: Transcript-level benchmark of the simulated data

Description Usage Arguments Value

View source: R/function.simulated.R

Description

Loads and processes the RNAseq data before comparing it with the real foldchanges. Will produce a number of benchmarking plots and files in the current working directory.

Usage

1
compareSimulated(ANALYSIS_NAME, txfile = "simulated.quant")

Arguments

ANALYSIS_NAME

A string indicating the name of the analysis/pipeline. Will be used in filenames, plot titles, etc.

txfile

The path to the transcript-level RNAseq expression matrix. If not given, will look for relevant files in the working directory. The expression matrix should have gene symbols in the first column/row.names, and sample names (either 's1', 's2', etc., or '1' '2', etc..) as column headers.

qt

A string indicating the unit of the expression matrix (either "FPKM", "TPM" or "COUNTS").

normMethod

The normalization method to use (see donorm). Defaults to 'TMM'.

uniquelyMappableLengths

Logical, whether to use uniquely mappable length, rather than full length, for FPKM calculation. Default TRUE.

requireAll

logical, whether all samples are required to proceed.

Value

Nothing, but produces many files in the working directory...


plger/RNAontheBENCH documentation built on May 25, 2019, 8:22 a.m.