plot.perturbationChanges: Operations on a 'perturbationChanges' object

In cTRAP: Identification of candidate causal perturbations from differential gene expression data

Description

Operations on a perturbationChanges object

Usage

## S3 method for class 'perturbationChanges'
plot(
  x,
  perturbation,
  input,
  method = c("spearman", "pearson", "gsea"),
  geneSize = 150,
  genes = c("both", "top", "bottom"),
  ...,
  title = NULL
)

## S3 method for class 'perturbationChanges'
x[i, j, drop = FALSE, ...]

## S3 method for class 'perturbationChanges'
dim(x)

## S3 method for class 'perturbationChanges'
dimnames(x)

Arguments

x	perturbationChanges object
perturbation	Character (perturbation identifier) or a similarPerturbations table (from which the respective perturbation identifiers are retrieved)
input	Named numeric vector of differentially expressed genes whose names are gene identifiers and respective values are a statistic that represents significance and magnitude of differentially expressed genes (e.g. t-statistics); or character of gene symbols composing a gene set that is tested for enrichment in reference data (only used if method includes gsea)
method	Character: one or more methods to compare data (spearman, pearson or gsea)
geneSize	Numeric: number of top up-/down-regulated genes to use as gene sets to test for enrichment in reference data; if a 2-length numeric vector, the first index is the number of top up-regulated genes and the second index is the number of down-regulated genes used to create gene sets; only used if method includes gsea and if input is not a gene set
genes	Character: when plotting gene set enrichment analysis (GSEA), plot most up-regulated genes (genes = "top"), most down-regulated genes (genes = "bottom") or both (genes = "both"); only used if method = "gsea" and geneset = NULL
...	Extra arguments
title	Character: plot title (if NULL, the default title depends on the context; ignored when plotting multiple perturbations)
i, j	Character or numeric indexes specifying elements to extract
drop	Boolean: coerce result to the lowest possible dimension?

Value

Subset, plot or return dimensions or names of a perturbationChanges object

See Also

Other functions related with the ranking of CMap perturbations: as.table.referenceComparison(), filterCMapMetadata(), getCMapConditions(), getCMapPerturbationTypes(), loadCMapData(), loadCMapZscores(), parseCMapID(), plot.referenceComparison(), plotTargetingDrugsVSsimilarPerturbations(), prepareCMapPerturbations(), print.similarPerturbations(), rankSimilarPerturbations()

Examples

data("diffExprStat")
data("cmapPerturbationsKD")

compareKD <- rankSimilarPerturbations(diffExprStat, cmapPerturbationsKD)
EIF4G1knockdown <- grep("EIF4G1", compareKD[[1]], value=TRUE)
plot(cmapPerturbationsKD, EIF4G1knockdown, diffExprStat, method="spearman")
plot(cmapPerturbationsKD, EIF4G1knockdown, diffExprStat, method="pearson")
plot(cmapPerturbationsKD, EIF4G1knockdown, diffExprStat, method="gsea")

data("cmapPerturbationsCompounds")
pert <- "CVD001_HEPG2_24H:BRD-A14014306-001-01-1:4.1"
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="spearman")
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="pearson")
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="gsea")

# Multiple cell line perturbations
pert <- "CVD001_24H:BRD-A14014306-001-01-1:4.1"
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="spearman")
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="pearson")
plot(cmapPerturbationsCompounds, pert, diffExprStat, method="gsea")

cTRAP documentation built on Nov. 8, 2020, 10:58 p.m.