C316.melt.lr: Melting Curves (resolution: 0.5 degrees Celsius / step) for...
In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data
Description
Usage
Format
Details
Source
References
Examples
Description
A melting curve for adk was performed.
Usage
1
data("C316.melt.lr")
Format
A data frame with 40 observations on the following 97 variables. The first
column ("T") contains the temperature (resolution: 0.5 degrees Celsius / step) and consecutive columns
contain the replicates ("A01" to "H12").
Details
adk was amplified in the Bio-Rad iQ5. After PCR, the temperature-dependent
change of fluorescence was simultaneously monitored (EvaGreen, Mao et al. 2007).
The primer sequences for adk were taken from this study.
adk fw: CTCAGGCTCAGTTCATCATGGA
adk rv: AGTTTGCCAGCATCCATAATGTC
PCR conditions: C316.amp
Source
Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt,
M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the
implication of its physicochemical properties for qPCR applications.
BMC Biotechnol. 7, 76.
Examples
1
2
chipPCR documentation built on March 5, 2021, 9:06 a.m.
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Description Usage Format Details Source References Examples
Description
A melting curve for adk was performed.
Usage
1 | data("C316.melt.lr")
|
Format
A data frame with 40 observations on the following 97 variables. The first column ("T") contains the temperature (resolution: 0.5 degrees Celsius / step) and consecutive columns contain the replicates ("A01" to "H12").
Details
adk was amplified in the Bio-Rad iQ5. After PCR, the temperature-dependent change of fluorescence was simultaneously monitored (EvaGreen, Mao et al. 2007). The primer sequences for adk were taken from this study.
adk fw: CTCAGGCTCAGTTCATCATGGA adk rv: AGTTTGCCAGCATCCATAATGTC
PCR conditions: C316.amp
Source
Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol. 7, 76.
Examples
1 2 |
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Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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