C317.amp: qPCR Experiment for the Amplification of adk Using the...
In chipPCR: Toolkit of Helper Functions to Pre-Process Amplification Data
Description
Usage
Format
Details
Source
References
Examples
Description
A quantitative real-time PCR of adk was performed.
Usage
1
data("C317.amp")
Format
A data frame with 40 observations on the following 97 variables. The first
column ("Cycle") contains the number of cycles and consecutive columns
contain the replicates ("A01" to "H12").
Details
adk was amplified in the Bio-Rad CFX96. The change of fluorescence was
simultaneously monitored (EvaGreen, Mao et al. 2007). The
primer sequences for adk were taken from this study.
gDNA: 28.43 ng/microL DNA concentration, 260/280 ratio= 1.96
adk fw: CTCAGGCTCAGTTCATCATGGA
adk rv: AGTTTGCCAGCATCCATAATGTC
PCR conditions:
10 minutes at 95 degrees Celsius
40 x
30 seconds at 95 degrees Celsius
45 seconds at 59 degrees Celsius
45 seconds at 68 degrees Celsius
Source
Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex
Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger,
P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt,
M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder.
Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the
implication of its physicochemical properties for qPCR applications.
BMC Biotechnol. 7, 76.
Examples
1
2
3
chipPCR documentation built on March 5, 2021, 9:06 a.m.
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Description Usage Format Details Source References Examples
Description
A quantitative real-time PCR of adk was performed.
Usage
1 | data("C317.amp")
|
Format
A data frame with 40 observations on the following 97 variables. The first column ("Cycle") contains the number of cycles and consecutive columns contain the replicates ("A01" to "H12").
Details
adk was amplified in the Bio-Rad CFX96. The change of fluorescence was simultaneously monitored (EvaGreen, Mao et al. 2007). The primer sequences for adk were taken from this study.
gDNA: 28.43 ng/microL DNA concentration, 260/280 ratio= 1.96
adk fw: CTCAGGCTCAGTTCATCATGGA adk rv: AGTTTGCCAGCATCCATAATGTC
PCR conditions: 10 minutes at 95 degrees Celsius 40 x 30 seconds at 95 degrees Celsius 45 seconds at 59 degrees Celsius 45 seconds at 68 degrees Celsius
Source
Stefan Roediger, Claudia Deutschmann, Claudia Zelck (BTU Cottbus - Senftenberg)
References
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33–74, 2013.
Mao, F., Leung, W.-Y., Xin, X., 2007. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol. 7, 76.
Examples
1 2 3 |
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