Description Usage Format Examples
Highly replicate number amplification curves with an approximate amplification efficiency of 87.5 percent at cycle number 18. The data were derived from a simulation such as the AmpSim function.
1 |
A data frame with 40 (Cycles) observations on the following 1000 (amplification curves) variables. The columns are all replicates.
1 2 3 4 5 6 7 8 9 10 11 12 | data(Eff875)
plot(NA, NA, xlim = c(1,40), ylim = c(0,max(Eff875)), xlab = "Cycle",
ylab = "RFU",
main = "Amplification Curves with 87.5 Percent Efficiency")
apply(Eff875[, 1:ncol(Eff875)], 2, function(x) lines(1:40,x))
Fmean <- rowMeans(Eff875[, 1:ncol(Eff875)])
lines(1:40, Fmean, col = "red", lwd = 3)
legend(1, quantile(unlist(Eff875), 0.9), c("Raw", "Mean"),
pch = c(19,19), col = c(1,2))
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