Eff1000: Highly Replicate Number Amplification Curves

Description Usage Format Examples

Description

Highly replicate number amplification curves with an approximate amplification efficiency of 100 percent at cycle number 18. The data were derived from a simulation such as the AmpSim function.

Usage

1

Format

A data frame with 40 (Cycles) observations on the following 1000 (amplification curves) variables. The columns are all replicates.

Examples

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data(Eff1000)

plot(NA, NA, xlim = c(1,40), ylim = c(0,max(Eff1000)), xlab = "Cycle",
    ylab = "RFU", 
    main = "Amplification Curves with 100 Percent Efficiency")
apply(Eff1000[, 1:ncol(Eff1000)], 2, function(x) lines(1:40,x))

Fmean <- rowMeans(Eff1000[, 1:ncol(Eff1000)])
lines(1:40, Fmean, col = "red", lwd = 3)

legend(1, quantile(unlist(Eff1000), 0.9), c("Raw", "Mean"), 
	pch = c(19,19), col = c(1,2))

chipPCR documentation built on March 5, 2021, 9:06 a.m.