View source: R/RNASeqPipelineR.R
Pre-run Checklist 1. Are the contig names consistent betwen BAM, Refrence and GTF file? 2. Is the BAM indexed? we index the bam files in the prepareBamFiles 3. Is you reference Indexed? We index the refrence genome before runing the tophat 4. Dose the reference genome have a dict file? we generated the dict file in the createGenomeDict
1 2 3 | qualityRNASeQC(picard.path = "/home/jdeng/bin/picard-tools-1.110/",
ncores = 6, rna_seqc.path = "/home/jdeng/bin/RNA-SeQC_v1.1.7.jar",
paired = FALSE)
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picard.path |
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ncores |
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rna_seqc.path |
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paired |
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