limma_pipe: Pipeline for limma+voom analysis

Description Usage Arguments Value

View source: R/limma_pipe.R

Description

Function to perform entire limma+voom analysis using voom with quality weights, and beginning with a SummarizedExperiment and ending with list of differential genes and generation of diagnostic plots along the way.

Usage

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limma_pipe(se = NULL, design = NULL, contrast = NULL,
  block.column = NULL, norm.method = "TMM", adjust.method = "BH",
  p.value = 0.05)

Arguments

se

SummarizedExperiment with an assay slot named counts_fil containing the filtered counts and colData slot containing sample metadata.

design

A design matrix specifying the experimental design. If NULL, then a design matrix will be created using the values for contrast.levels and block.levels.

contrast

A matrix of contrasts (as created by makeContrasts in the limma package specifying the comparisons to perform). If NULL, then will be created from the design matrix.

block.column

The column in the samples metadata dataframe specifying the block/additive effect column. Ignored if design is not NULL.

norm.method

Normalisation method to use - may be "TMM", "RLE", "upperquartile" or "none".

adjust.method

Method to be used for adjustment of nominal p-values. May be one of "BH", "bonferroni", "holm", "hochberg", "hommel", "BY".

p.value

Value between 0 and 1. Adjusted p-value for the differential expression analysis.

Value

Results of limma+voom differential analysis.


anilchalisey/parseR documentation built on May 7, 2019, 7:45 a.m.