QuantifyTcrClones: Append Clone Properties

In bimberlabinternal/CellMembrane: A package with high-level wrappers and pipelines for single-cell RNA-seq tools

Append Clone Properties

Description

A function to append clone properties to a Seurat object

Usage

QuantifyTcrClones(seuratObj, tcrClonesFile, groupingFields = "cDNA_ID")

Arguments

seuratObj	The Seurat object to append clone properties to
tcrClonesFile	The 10x clonotypes file for this Seurat object. Single lanes can use the CellRanger/Vloupe contigs CSV file. Merged lanes need to merge these files and modify them to create unique cellbarcodes and clonotype names, such as the code from Rdiscvr::CreateMergedTcrClonotypeFile().
groupingFields	A list of fields used to group cells by sample, which establishes librarySize

bimberlabinternal/CellMembrane documentation built on Dec. 25, 2024, 8:10 a.m.