genesbyexpressionpercentile: Find genes expressed in early ring, late ring, early...

Description Arguments Value

Description

Find genes expressed in early ring, late ring, early trophozoite, late trophozoite, early schizont, late schizont, merozoite, sporozoite, or gametocyte. <br><br>

The data is from a total of 17 microarray experiments (1) performed using an Affymetrix short oligonucleotide platform. Cultures were synchronized using two independent methods, 5% D-sorbitol and temperature cycling, for all developmental stages except Gametocyte and Sporozoite. This platform contains oligos (25-mers) derived from the predicted coding regions of the entire <I>P. falciparum</I> genome. <P>Expression values for each probe set were obtained by the MOID algorithm (2). Queries can be performed to retrieve only those genes in at least the Xth percentile level, where X can be from 95 to 5. In this way it is possible to select only the most highly expressed genes, to any desired level above the 5th percentile. Genes are returned in decreasing order of their intensity. <p>However, please note that, regardless of the percentile expression level selected, only genes that are above background and whose observed expression level would not be expected by chance (MOID expression level >10 and logP < -0.5) are included and will be reported. This means that genes that have only a single probe on the array will not be reported as their logP values will always be too high to satisfy the query.

Arguments

extraerythrocytic_stages

Select the extraerythrocytic life cycle stage, for which the genes are to be fetched. Provide one or more values. Use comma as a delimter.

intraerythrocytic_stages

Select the intraerythrocytic life cycle stage, for which the genes are to be fetched. Provide one or more values. Use comma as a delimter.

min_expression_percentile

Lower bound on expression percentile.

max_expression_percentile

Upper bound on expression percentile.

o-fields

Single valued attributes of the feature. Provide one or more values. Use comma as a delimter.

o-tables

Multi-valued attributes of the feature. Provide one or more values. Use comma as a delimter.

.convert

a logical value or a function that controls how the result of the method is returned. If this is a function, the character string or raw vector is passed to this function and it converts it appropriately. If this is a logical value and TRUE, then we attempt to convert the result based on its Content-Type returned by the Web server. If this is FALSE, the value from the Web server is returned as is.

.url

the URL for the Web request. This defaults to the correct value, but can be specified by the caller if the method is available at a different URL, e.g. locally or in a mirror server.

.json

a logical value controlling whether to use the JSON or the XML version of the method

Value

text/xml text/plain


duncantl/REuPathDB documentation built on May 15, 2019, 5:28 p.m.