genesbymicroarrpagelmajorpct: Find <i>L. major</i> genes based on your specified expression...

In duncantl/REuPathDB: Interface to REST API of (EuPathDB)

Description

Find <i>L. major</i> genes based on your specified expression percentile and lifecycle stage comparison. <br><br>Total RNA was prepared from <i>Leishmania major</i> strain Friedlin clone V1 (provided by David Sacks, NIH). Stages used were early log phase procyclic promastigotes (P) , metacyclics prepared by negative selection with peanut agglutinin (M), or amastigotes (A) purified from lesions obtained following footpad inoculations of susceptible BALB/c mice. RNAs were converted to cDNA using dT priming and labeled with appropriate fluors. Microarrays consisted of 70 mers spotted on glass slides. Three independent biological replicas were performed, each with 12 technical replicas. The procyclic promastigote sample was taken as the reference and comparisons of the metacyclic or amastigotes are provided. (This query returns a column for the average fold change for the biological replicas and the individual data points are provided as a graph in the gene page.)

Arguments

PaGE_comparisons_pct_Lm	Choose a comparison Provide one or more values. Use comma as a delimter.
min_expression_percentile	Lower bound on expression percentile.
max_expression_percentile	Upper bound on expression percentile.
protein_coding_only	Should only protein coding genes be returned?
o-fields	Single valued attributes of the feature. Provide one or more values. Use comma as a delimter.
o-tables	Multi-valued attributes of the feature. Provide one or more values. Use comma as a delimter.
.convert	a logical value or a function that controls how the result of the method is returned. If this is a function, the character string or raw vector is passed to this function and it converts it appropriately. If this is a logical value and TRUE, then we attempt to convert the result based on its Content-Type returned by the Web server. If this is FALSE, the value from the Web server is returned as is.
.url	the URL for the Web request. This defaults to the correct value, but can be specified by the caller if the method is available at a different URL, e.g. locally or in a mirror server.
.json	a logical value controlling whether to use the JSON or the XML version of the method

Value

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duncantl/REuPathDB documentation built on May 15, 2019, 5:28 p.m.