genesbymicroarrpagelinfantumpct: Find L. infantum genes based on your specified...
In duncantl/REuPathDB: Interface to REST API of (EuPathDB)

Description Arguments Value

Find L. infantum genes based on your specified expression percentile and lifecycle stage. (Transcriptional analysis of promastigote compared to intracellular amastigote and axenic amastigote.) In this study, DNA oligonucleotide full-genome arrays representing all L. infantum annotated genes were used to determine global RNA expression profiling of axenic amastigotes and to assess how it compares to intracellular amastigotes. The full-genome DNA microarrays included one 70-oligonucleotides probe for each gene of L. infantum. Intracellular amastigote analysis was a two-condition experiment, comparing promastigote stage to intracellular amastigote stage. This included six biological replicates for each stage, independently grown and harvested. One replicate per array. Axenic amastigote analysis was a two-condition experiment, comparing promastigote stage to axenic amastigote stage. This included four biological replicates for each stage, independently grown and harvested. One replicate per array. Promastigote cells were grown in SDM-79 medium supplemented with 10% heat-inactivated fetal bovine serum and 5 micrograms/ml hemin at 25'C. For intercellular amastigotes, amastigote cells were grown in THP-1 macrophage in RMPI 1640 medium supplemented with 10% heat-inactivated fetal bovine. For axenic amastigotes, amastigote cells were isolated from infected mice or were grown in MAA20 medium supplemented with 20% heat-inactivated fetal bovine serum and 15 micrograms/ml hemin at 37'C. For further details please refer: <a href="http://www.ncbi.nlm.nih.gov/pubmed/19393160">Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum</a>

`PaGE_comparisons_pct_Li`	Choose a comparison Provide one or more values. Use comma as a delimter.
`min_expression_percentile`	Lower bound on expression percentile.
`max_expression_percentile`	Upper bound on expression percentile.
`protein_coding_only`	Should only protein coding genes be returned?
`o-fields`	Single valued attributes of the feature. Provide one or more values. Use comma as a delimter.
`o-tables`	Multi-valued attributes of the feature. Provide one or more values. Use comma as a delimter.
`.convert`	a logical value or a function that controls how the result of the method is returned. If this is a function, the character string or raw vector is passed to this function and it converts it appropriately. If this is a logical value and `TRUE`, then we attempt to convert the result based on its Content-Type returned by the Web server. If this is `FALSE`, the value from the Web server is returned as is.
`.url`	the URL for the Web request. This defaults to the correct value, but can be specified by the caller if the method is available at a different URL, e.g. locally or in a mirror server.
`.json`	a logical value controlling whether to use the JSON or the XML version of the method