genesbygametocyteexpression: Find genes by percentile protein expression in gametocytes.

Description Arguments Value

Description

Find <i>Plasmodium falciparum</i> genes expressed during gametocytogenesis in strain NF54, strain 3D7, or MACS-purified strain 3D7. It uses data from a total of 23 microarray experiments (1), as shown here for gene PFD0830w:<br><br> <div align="center"> <img src="images/plasmo_gam_pct_pfd0830w.png"> </div><br><br> These experiments were performed using an Affymetrix short oligonucleotide platform, which contains oligos (25-mers) derived from the predicted coding regions of the entire <I>P. falciparum</I> genome. </P> <P> Expression values for each probe set were obtained by the MOID algorithm (2). Genes can be found by their percentile expression in the selected set of assays.<br><br> However, please note that, regardless of the percentile expression level selected, only genes that are above background and whose observed expression level would not be expected by chance (MOID expression level >10 and logP < -0.5) are included and will be reported. This means that genes that have only a single probe on the array will not be reported as their logP values will always be too high to satisfy the query. <br><br> <pre><b>References:</b><br>(1) Jason A. Young, Quinton L. Fivelman, Peter L. Blair, Patricia de la Vega,<br>Karine G. Le Roch, Yingyao Zhou, Daniel J. Carucci, David A. Baker, Elizabeth A. Winzeler<br><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16005087&dopt=Citation">The Plasmodium falciparum sexual development transcriptome: a microarray analysis using ontology-based pattern identification</a><br>Mol Biochem Parasitol. 2005 Jul 5; <br>(2) Zhou Y, Abagyan R.<br><a href="http://www.biomedcentral.com/1471-2105/3/3">Match-Only Integral Distribution (MOID) Algorithm for high-density oligonucleotide array analysis.</a><br>BMC Bioinformatics. 2002;3(1):3.</pre>

Arguments

day_and_strain

Choose one or more strain/day combination Provide one or more values. Use comma as a delimter.

min_expression_percentile

Lower bound on expression percentile.

max_expression_percentile

Upper bound on expression percentile.

o-fields

Single valued attributes of the feature. Provide one or more values. Use comma as a delimter.

o-tables

Multi-valued attributes of the feature. Provide one or more values. Use comma as a delimter.

.convert

a logical value or a function that controls how the result of the method is returned. If this is a function, the character string or raw vector is passed to this function and it converts it appropriately. If this is a logical value and TRUE, then we attempt to convert the result based on its Content-Type returned by the Web server. If this is FALSE, the value from the Web server is returned as is.

.url

the URL for the Web request. This defaults to the correct value, but can be specified by the caller if the method is available at a different URL, e.g. locally or in a mirror server.

.json

a logical value controlling whether to use the JSON or the XML version of the method

Value

text/xml text/plain


duncantl/REuPathDB documentation built on May 15, 2019, 5:28 p.m.