Find <i>Plasmodium falciparum</i> genes expressed during gametocytogenesis in strain NF54, strain 3D7, or MACS-purified strain 3D7. It uses data from a total of 23 microarray experiments (1), as shown here for gene PFD0830w:<br><br> <div align="center"> <img src="images/plasmo_gam_pct_pfd0830w.png"> </div><br><br> These experiments were performed using an Affymetrix short oligonucleotide platform, which contains oligos (25-mers) derived from the predicted coding regions of the entire <I>P. falciparum</I> genome. </P> <P> Expression values for each probe set were obtained by the MOID algorithm (2). Genes can be found by their percentile expression in the selected set of assays.<br><br> However, please note that, regardless of the percentile expression level selected, only genes that are above background and whose observed expression level would not be expected by chance (MOID expression level >10 and logP < -0.5) are included and will be reported. This means that genes that have only a single probe on the array will not be reported as their logP values will always be too high to satisfy the query. <br><br> <pre><b>References:</b><br>(1) Jason A. Young, Quinton L. Fivelman, Peter L. Blair, Patricia de la Vega,<br>Karine G. Le Roch, Yingyao Zhou, Daniel J. Carucci, David A. Baker, Elizabeth A. Winzeler<br><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16005087&dopt=Citation">The Plasmodium falciparum sexual development transcriptome: a microarray analysis using ontology-based pattern identification</a><br>Mol Biochem Parasitol. 2005 Jul 5; <br>(2) Zhou Y, Abagyan R.<br><a href="http://www.biomedcentral.com/1471-2105/3/3">Match-Only Integral Distribution (MOID) Algorithm for high-density oligonucleotide array analysis.</a><br>BMC Bioinformatics. 2002;3(1):3.</pre>
day_and_strain |
Choose one or more strain/day combination Provide one or more values. Use comma as a delimter. |
min_expression_percentile |
Lower bound on expression percentile. |
max_expression_percentile |
Upper bound on expression percentile. |
o-fields |
Single valued attributes of the feature. Provide one or more values. Use comma as a delimter. |
o-tables |
Multi-valued attributes of the feature. Provide one or more values. Use comma as a delimter. |
.convert |
a logical value or a function that controls how the result of the method is returned. If this is a function, the character string or raw vector is passed to this function and it converts it appropriately. If this is a logical value and |
.url |
the URL for the Web request. This defaults to the correct value, but can be specified by the caller if the method is available at a different URL, e.g. locally or in a mirror server. |
.json |
a logical value controlling whether to use the JSON or the XML version of the method |
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