Description Usage Arguments Value
View source: R/SingleR.Create.R
Wrapper function to create a SingleR object
1 2 3 4 5 6 | CreateSinglerObject(counts, annot = NULL, project.name, min.genes = 0,
technology = "10X", species = "Human", citation = "",
ref.list = list(), normalize.gene.length = F,
variable.genes = "de", fine.tune = T, do.signatures = F,
clusters = NULL, do.main.types = T, reduce.file.size = T,
temp.dir = NULL, numCores = SingleR.numCores)
|
counts |
a tab delimited text file containing the counts matrix, a 10X directory name or a matrix with the counts. |
annot |
a tab delimited text file or a data.frame. Rownames correspond to column names in the counts data |
project.name |
the project name |
min.genes |
Include cells where at least this many genes are detected (number non-zero genes). |
technology |
The technology used for creating the single-cell data. |
species |
The species of the sample ('Human' or 'Mouse'). |
citation |
a citation for the project. |
ref.list |
a list of reference objects. If NULL uses the predefined reference objects - Mouse: ImmGen and Mouse.RNAseq, Human: HPCA and Blueprint+Encode. |
normalize.gene.length |
if a full-length method set to TRUE, if a 3' method set to FALSE. |
variable.genes |
variable gene method to use - 'sd' or 'de'. Default is 'de'. |
fine.tune |
perform fine tuning. Default is TRUE. Fine-tuning may take long to run. |
do.signatures |
create signatures data |
clusters |
input cluster id for each of the cells with at least min.genes, if NULL uses SingleR clusterings. |
do.main.types |
run the SingleR pipeline for main cell types (cell types grouped together) as well. |
reduce.file.size |
remove less used SingleR fields that increase the object size. |
temp.dir |
used by the SingleR webtool. |
numCores |
Number of cores to use. |
a SingleR object
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.