# Discover differentially abundant time intervals

### Description

Calculate time intervals of significant differential abundance. Currently only one method is implemented (ssanova). fitSSTimeSeries is called with method="ssanova".

### Usage

1 2 3 |

### Arguments

`obj` |
metagenomeSeq MRexperiment-class object. |

`formula` |
Formula for ssanova. Of the form: abundance ~ ... where ... includes any pData slot value. |

`feature` |
Name or row of feature of interest. |

`class` |
Name of column in phenoData of MRexperiment-class object for class memberhip. |

`time` |
Name of column in phenoData of MRexperiment-class object for relative time. |

`id` |
Name of column in phenoData of MRexperiment-class object for sample id. |

`method` |
Method to estimate time intervals of differentially abundant bacteria (only ssanova method implemented currently). |

`lvl` |
Vector or name of column in featureData of MRexperiment-class object for aggregating counts (if not OTU level). |

`include` |
Parameters to include in prediction. |

`C` |
Value for which difference function has to be larger or smaller than (default 0). |

`B` |
Number of permutations to perform. |

`norm` |
When aggregating counts to normalize or not. |

`log` |
Log2 transform. |

`sl` |
Scaling value. |

`...` |
Options for ssanova |

### Value

List of matrix of time point intervals of interest, Difference in abundance area and p-value, fit, area permutations, and call.

A list of objects including:

timeIntervals - Matrix of time point intervals of interest, area of differential abundance, and pvalue.

data - Data frame of abundance, class indicator, time, and id input.

fit - Data frame of fitted values of the difference in abundance, standard error estimates and timepoints interpolated over.

perm - Differential abundance area estimates for each permutation.

call - Function call.

### See Also

`cumNorm`

`fitSSTimeSeries`

`plotTimeSeries`

### Examples

1 2 3 |

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